Table 1.
Comparison of the apparent Michaelis-Menten constant (Km) and catalytic constant (Kcat) of the NCs-FA nanoprobes, HRP, Fe3O4 MNPs, BSA-Au NCs, Co3O4 NPs, GO-Au NCs and CGN.
| Catalyst | Substrate | Km (mM) |
Kcat (s-1) |
|---|---|---|---|
| Fe3O4 MNPs29 | TMB | 0.098 | 8.58×104 |
| Fe3O4 MNPs29 Co3O4 NPs42 |
H2O2 | 154 | 3.02×104 |
| TMB | 0.037 | 1.83×102 | |
| Co3O4 NPs42 | H2O2 | 140.07 | 3.53×102 |
| CGN43 | TMB | 0.12 | 2.48×104 |
| CGN43 | H2O2 | 245 | 2.13×104 |
| BSA-Au39 | TMB | 0.00253 | 7.24×104 |
| BSA-Au39 | H2O2 | 25.3 | 9.17×104 |
| GO-AuNCs44 GO-AuNCs44 |
TMB | 0.16 | 1.97×102 |
| H2O2 | 142.39 | 6.08×102 | |
| The NCs-FA nanoprobes | TMB | 0.00664 | 4.49×104 |
| The NCs-FA nanoprobes | H2O2 | 2.46 | 8.67×104 |
| HRP | TMB | 0.301 | 5.18×103 |
| HRP | H2O2 | 0.935 | 4.05×103 |