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. 2014 Jan 14;4(2):215–228. doi: 10.7150/thno.7868

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Membrane-bound COMT (MB-COMT) is a sensitive marker of early injury in the mouse model. (A) MB-COMT, along with RBP4, changed significantly one hour post-APAP treatment. ALT and AST levels were assayed enzymatically while RBP4, and MB-COMT were assayed by quantitative western blotting. Treatment values were relative to mock-treatment control values which were normalized to 100. (B) MB-COMT levels were elevated in both plasma and liver 24 hours after treatment with 300 mg/kg APAP, as determined by quantitative western blotting. (C) Liver lysate levels of MB-COMT but not soluble COMT (S-COMT) were maximal 24 and 48 hours after treatment, when injury was maximal. (D) Immunohistochemistry staining of MB-COMT 48 hours after treatment.