Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jan 24;8:7. doi: 10.3389/fncel.2014.00007

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014 Mòdol, Mancuso, Alé, Francos-Quijorna and Navarro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Analysis of KCC2 and phopho-KCC2 expression in the ventral spinal cord of SCI and sciatic nerve injured mice. (A) Representative blots of phopho-KCC2 and total KCC2 (on both monomeric and oligomeric states) in SCI and crush mice. (B) KCC2 western blots quantifications. Graphs represent the ratio between the oligomeric KCC2 (functional state) vs. monomeric KCC2 and the phopho-KCC2 (active form) vs. monomeric KCC2. Both analyses revealed a decrease in the active form of the KCC2 in SCI (28 days after injury) and in sciatic nerve crush (7 days after injury). Data are represented as mean ± s.e.m.; ^*p < 0.05, ^**p < 0.01 vs. naïve animals. (C) Confocal images of L4 spinal MNs revealed the presence of membrane-bound (active) KCC2 in naïve but not in SCI and sciatic nerve injured animals. Arrowheads point internalized KCC2 aggregates. Scale bar 20 μm.