Table 1. Comparative data base of ELISA and proposed SPR biosensor in low, mid and high positive patient samples of dengue virus.
| ELISA results | Biosensor results (ΔθSPR) | |||||||
|---|---|---|---|---|---|---|---|---|
| Type of sample (HI) | Patient serum | P/N ratio | NS1 | IgM | S.T. 1 | S.T. 2 | S.T. 3 | S.T. 4 |
| Low positive (Antibody titre 10–160) | AH01 | 2.48 | − | + | 1.0110 | 0.7454 | 0.5808 | 0.5923 |
| AH02 | 2.67 | − | + | 1.0570 | 0.7186 | 0.5845 | 0.6344 | |
| AH03 | 2.59 | + | + | 1.0688 | 0.7211 | 0.5785 | 0.6369 | |
| AH04 | 2.47 | + | + | 1.0013 | 0.6498 | 0.5499 | 0.5957 | |
| AH05 | 2.81 | + | + | 1.0102 | 0.6694 | 0.5162 | 0.5572 | |
| AH06 | 2.47 | + | + | 1.0060 | 0.6560 | 0.5080 | 0.5467 | |
| AH07 | 2.79 | + | + | 0.9665 | 0.6910 | 0.5548 | 0.6105 | |
| AH08 | 2.14 | + | + | 1.0019 | 0.7015 | 0.5002 | 0.5844 | |
| AH09 | 2.93 | − | + | 1.1015 | 0.7528 | 0.5775 | 0.6512 | |
| AH10 | 2.58 | − | + | 1.0104 | 0.7501 | 0.5771 | 0.6081 | |
| Mid positive (Antibody titre 160–640) | AH11 | 3.01 | + | + | 0.9983 | 0.8863 | 0.6866 | 0.7051 |
| AH12 | 3.93 | + | + | 1.0574 | 0.9095 | 0.5849 | 0.8077 | |
| AH13 | 3.45 | − | + | 1.1691 | 0.7965 | 0.5987 | 0.7498 | |
| AH14 | 3.75 | + | + | 0.9862 | 0.9380 | 0.6720 | 0.7899 | |
| AH15 | 3.34 | − | + | 1.1001 | 0.8754 | 0.6972 | 0.7386 | |
| AH16 | 4.59 | − | + | 1.1124 | 0.9566 | 0.7399 | 0.8211 | |
| AH17 | 4.5 | + | + | 1.1092 | 0.9487 | 0.7410 | 0.8222 | |
| AH18 | 4.96 | + | + | 1.1978 | 0.9637 | 0.7405 | 0.8230 | |
| AH19 | 4.34 | − | + | 1.0543 | 0.7688 | 0.7337 | 0.6968 | |
| AH20 | 4.63 | + | + | 1.1733 | 0.9598 | 0.7234 | 0.8176 | |
| High positive (Antibody titre 1280–10240) | AH21 | 5.25 | + | + | 1.1255 | 1.0114 | 0.7154 | 0.9045 |
| AH22 | 5.69 | + | + | 1.1469 | 0.9778 | 0.7740 | 0.8304 | |
| AH23 | 5.12 | + | + | 1.1344 | 0.9463 | 0.7595 | 0.7983 | |
| AH24 | 5.6 | + | + | 1.1226 | 0.9462 | 0.7519 | 0.8084 | |
| AH25 | 6.53 | − | + | 1.1215 | 0.9084 | 0.7585 | 0.7845 | |
| AH26 | 7.13 | − | + | 1.1987 | 1.0695 | 0.7021 | 0.8793 | |
| AH27 | 7.32 | + | + | 1.2731 | 1.1128 | 0.8676 | 0.9519 | |
| AH28 | 6.06 | + | + | 1.1996 | 0.9954 | 0.7567 | 0.8922 | |
| AH29 | 7.02 | − | + | 1.2453 | 1.0532 | 0.8063 | 0.9476 | |
| AH30 | 7.38 | − | + | 1.2782 | 0.9869 | 0.7631 | 0.8541 | |