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. 2014 Jan 24;4:3883. doi: 10.1038/srep03883

Figure 5. The therapeutic effects of miR-126 transfer by mi-BLs and US exposure on mice with hindlimb ischemia.

Figure 5

Ten days after femoral artery ligation, mice were treated with mi-BLs and US. The treatment was administered via tail vein injection twice daily every two days to mice with hindlimb ischemia. We injected a solution of miRNA (40 μg of miCont or miR-126) and BLs (200 μg). (a) miR-126 levels in the ischemic hindlimb after transfection. (b), (c) The effect of miR-126 transfer by mi-BLs and US on mRNA expression for angiogenic genes. Two (b) or seven (c) days after the second transfection, RNA was isolated from the thigh muscle and analyzed using real-time PCR. (d) The effect of miR-126 transfer using mi-BLs and US on the recovery of blood flow. After the second transfection, blood flow was measured using a laser Doppler blood flow meter. All data are reported as the mean ± SD (n = 4–6). * indicates P < 0.05 using a one-way ANOVA with Tukey's post-hoc test.