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. 2014 Jan 24;4:3861. doi: 10.1038/srep03861

Figure 2. Reversible tetramerisation and further higher-order association of the polysaccharide 6-deoxy-6-(ω-aminoethyl)aminocellulose2 AEA-1.

Figure 2

Top: Monomer unit of degree of polymerization ~10, degree of substitution at C-6 DSAmine = 0.83 and degree of substitution at C-2 of tosyl residues DSTosyl = 0.2, yielding an M ~ 3250 Da and s ~ 0.5 S. Middle: Assembly into tetramers with M ~ 13000 Da and s ~ 1.7 S. Lower: Sedimentation coefficient distribution for AEA-1 at different concentrations 2.0 mg/ml (black), 1.0 mg/ml (red) and 0.75 mg/ml (blue). Based on the s ~ M2/3 scaling relationship the super-monomers associate into super-trimers, super-hexamers and super-9-mers with evidence also for some super-dimers, although the latter were not evident at the highest loading concentration. The proportion of the super-monomers drops relative to the higher order species showing partial reversibility even with the higher-order association.