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. 2014 Jan 24;4:3846. doi: 10.1038/srep03846

Figure 2. ARRDC3 expression is suppressed epigenetically in BLBC cells.

Figure 2

(a) MDA-MB-231(top panel) and MCF-7 cells (bottom panel) were treated with DMSO or EtOH (control) and HDAC inhibitors (NIA or TSA) for 24 hr at indicated concentrations. (b) MDA-MB-231(upper panel) and MCF-7cells (lower panel) were treated with DMSO (control) and DNMT inhibitor (5-Aza-dC) for 48 hr at indicated concentrations. (c) MDA-MB-231cells were pre-treated with DMSO or DNMT inhibitor, 5-Aza-dC (200 nM and 500 nM) for 24 hr, followed by HDAC inhibitors (5 mM NIA and 1 μM TSA) treatment for 24 hr. The lysates from cells mentioned above were prepared and used for Western blotting analysis with anti-ARRDC3 and β-actin (loading control) antibodies. All western blot results presented were carried out at least 3 times.