FIGURE 1.
The accR gene is required for CCR control of the PN promoter. Azoarcus sp. CIB (CIB), its AccR null counterpart (CIBΔaccR), and CIBΔaccR harboring plasmid pBBR5AccR, which expresses AccR from the Plac promoter (CIBΔaccR/accR), were grown anaerobically in minimal medium supplemented with 3 mm benzoate (black bars) or a mixture of 3 mm benzoate and 0.4% succinate (white bars). Total RNA was isolated from cells grown until mid-exponential phase, and the activity of the PN promoter (in arbitrary units) was measured by real-time RT-PCR. Graphed values are the average of three independent experiments ± S.D. (error bars).