FIGURE 3.

DNA binding by AccR is phosphorylation-dependent. Shown are gel retardation analyses of the P_N probe by AccR derivatives (0–12 μm) as described under “Experimental Procedures.” A, assays with increasing concentrations of untreated His₆-AccR protein (AccR). Free P_N probe is indicated by an arrow. B, assays performed with increasing concentrations of carbamoyl phosphate-treated His₆-AccR (AccR-P). Free P_N probe and the major P_N·AccR-P complex are indicated by arrows. C, assays performed with increasing concentrations of His₆-AccRD60E (AccRD60E). Free P_N probe and the major P_N·AccRD60E complex are indicated by arrows.