FIGURE 5.
AccR-P represses transcription from the PN promoter in vitro. Multiple-round in vitro transcription reactions, as detailed under “Experimental Procedures,” contained 5 nm supercoiled pJCD-PN plasmid DNA (which produces a 184-nucleotide mRNA from PN and a 105-nucleotide control mRNA), 40 nm E. coli RNA polymerase, and 200 nm His6-Fnr* activator. Reactions were carried out in the absence of AccR or in the presence of increasing concentrations of phosphorylated His6-AccR (AccR-P) or unphosphorylated His6-AccR (AccR).