FIGURE 6.

CAccR functions as an independent monomeric DNA binding domain. A, gel retardation analysis of the P_N probe, as under Fig. 3A, using increasing concentrations (0–10 μm) of His₆-CAccR (CAccR). The free P_N probe and the major P_N·CAccR complex are indicated by arrows. B, sedimentation coefficient distribution c(s) corresponding to 10 μm His₆-CAccR is represented in relation to the sedimentation coefficient (S). The peak corresponding to the single His₆-CAccR species detected is indicated. C, sedimentation equilibrium data of His₆-CAccR (circles) and best fit analysis assuming a theoretical protein monomer (solid line) or dimer (dashed line).