FIGURE 1.

Transgenic overexpression of G0S2 in adipose tissue. A, targeting scheme for tissue-specific G0S2 overexpression. B, cDNA synthesized from various tissues in aP2-G0S2 mice was subjected to PCR using aP2-G0S2 primers. PCR products were resolved on a 1.5% agarose gel by electrophoresis. C, immunoblotting analysis of G0S2 expression in gonadal WAT and BAT. β-Actin was used as a loading control. D, body weight measurement on 12-week-old male and female mice (n = 8 per group). E, food intake as measured by g/day (n = 12 per group) from 12-week-old female mice.