TABLE 1.
Sequences and thermal stabilities of peptides used in this study
SSP, single-stranded peptide; NA, not applicable.
| Peptide | Sequence | Tm |
|---|---|---|
| °C | ||
| Knight SSP | Lys(Mca)-Pro-Leu-Gly↓Leu-Lys(Dnp)-Ala-Arg-NH2 | NA |
| fTHP-15 | (Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | 55 |
| GDK A | Gly-Asp-Lys-(Gly-Pro-Hyp)4-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)4-Gly-Asp-Lys-NH2 | 47 |
| GDK B | Gly-Pro-Hyp-Gly-Asp-Lys-(Gly-Pro-Hyp)3-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)3-Gly-Asp-Lys-Gly-Pro-Hyp-NH2 | 37 |
| GDK C | (Gly-Pro-Hyp)2-Gly-Asp-Lys-(Gly-Pro-Hyp)2-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)2-Gly-Asp-Lys-(Gly-Pro-Hyp)2-NH2 | 40 |
| GDK D | (Gly-Pro-Hyp)3-Gly-Asp-Lys-Gly-Pro-Hyp-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-Gly-Pro-Hyp-Gly-Asp-Lys-(Gly-Pro-Hyp)3-NH2 | 37 |
| GDK E | (Gly-Pro-Hyp)4-Gly-Asp-Lys-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-Gly-Asp-Lys-(Gly-Pro-Hyp)4-NH2 | 27 |
| KGD A | Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)4-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)3-Gly-Pro-Lys-Gly-Asp-Hyp-NH2 | 52 |
| KGD B | Gly-Pro-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)3-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)2-Gly-Pro-Lys-Gly-Asp-Hyp-Gly-Pro-Hyp-NH2 | 51 |
| KGD C | Gly-Pro-Hyp-Gly-Pro-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)2-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-Gly-Pro-Hyp-Gly-Pro-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)2-NH2 | 51 |
| KGD D | (Gly-Pro-Hyp)2-Gly-Pro-Lys-Gly-Asp-Hyp-Gly-Pro-Hyp-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-Gly-Pro-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)3-NH2 | 51 |
| KGD E | (Gly-Pro-Hyp)3-Gly-Pro-Lys-Gly-Asp-Hyp-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)4-NH2 | 43 |
| α1(III)748–798 fTHP | (Gly-Pro-Hyp)7-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | 64 |
| α1(III)748–798 KGD fTHP | Gly-Pro-Lys-Gly-Asp-Hyp-(Gly-Pro-Hyp)5-Gly-Pro-Lys(Mca)-Gly-Pro-Gln-Gly↓Leu-Arg-Gly-Gln-Lys(Dnp)-Gly-Val-Arg-(Gly-Pro-Hyp)5-NH2 | 60 |