TABLE 3.

Kinetic parameters for hydrolysis of Gly-Asp-Lys triple-helical substrates by MMPs

Peptide	MMP-2		MMP-8		MMP-9		MMP-12		MMP-13		MT1-MMP
	Km	kcat	Km	kcat	Km	kcat	Km	kcat	Km	kcat	Km	kcat
	μm	s−1	μm	s−1	μm	s−1	μm	s−1	μm	s−1	μm	s−1
fTHP-15	5.4 ± 0.97	0.654	23.0 ± 9.4	0.500	6.4 ± 0.79	0.449	56.6 ± 0.50	0.111	21.8 ± 2.2	0.677	13.1 ± 1.6	0.300
GDK A	5.7 ± 0.79	1.485	19.4 ± 0.75	0.559	2.9 ± 0.52	2.363	26.3 ± 2.2	0.153	8.5 ± 1.12	0.289	6.9 ± 0.89	0.136
GDK B	4.5 ± 0.60	1.424	25.1 ± 2.1	1.180	2.3 ± 0.14	2.700	72.8 ± 6.3	0.554	5.9 ± 0.67	0.330	9.1 ± 0.86	0.305
GDK C	3.3 ± 0.35	2.374	18.6 ± 0.74	1.681	1.3 ± 0.22	2.621	57.4 ± 2.4	0.892	11.4 ± 0.59	1.213	8.3 ± 0.86	0.589
GDK D	2.7 ± 0.62	2.635	44.4 ± 2.4	5.758	1.7 ± 0.21	4.071	19.9 ± 3.4	0.361	25.6 ± 2.16	3.402	9.5 ± 0.29	1.026
GDK E	2.4 ± 0.42	2.804	58.5 ± 6.7	8.233	1.4 ± 0.15	2.679	32.1 ± 4.3	1.047	19.9 ± 2.0	4.197	12.0 ± 0.97	1.291