FIGURE 1.

Expression pattern of the SO₄²⁻/Cl⁻/OH⁻ exchanger Slc26a2. a, the indicated mouse tissues were used to analyze Slc26a2 mRNA by RT-PCR and protein by Western blot analysis. IB, immunoblot. b, immunolocalization of Slc26a2 in chondrocytes from mouse articular cartilage explants. c and d, changes in Slc26a2 mRNA during chondrocyte differentiation and maturation that were measured as a function of time in culture (c) and during cell passages (d). Col-II was used as the marker for proliferating chondrocytes and MMP13 as the marker for terminal chondrogenesis of hypertrophic chondrocytes in micromass culture of embryonic limb mesenchymal cells. Expression was analyzed by qPCR (columns) and RT-PCR (blots). The results are given as mean ± S.E. of three independent experiments.