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Detection of endogenous Kv7.4, Kv7.5, and TRP6 channels in MASMCs based on PLA. Images of MASMCs stained with mouse anti-KCNQ4 antibody (Kv7.4), rabbit anti-KCNQ5 antibody (Kv7.5), or rabbit anti-TRPC6 antibody (TRPC6). Ab ctrl, omission of the primary antibody. phase, phase contrast. PLA, PLA fluorescence signal. PLA/DAPI, merged PLA/DAPI signals. Merge, merged phase contrast/PLA/DAPI signals. Images are representative of 3 independent experiments. Scale bar: 10 μm.
