TABLE 4.

Summary of LC-ESI-MS/MS analysis of E. coli proteins glycosylated by ApNGT

Modified residues are underlined and in bold. (Annotated spectra are shown in supplemental Fig. S4.)

UniProt#	Name	Description	Observed m/z	Error	Mascot score	Peptide sequence
				ppm
P0A6Y8	DnaK	Chaperone protein	769.7334 (3+)	1.10	71.9	R.IINEPTAAALAYGLDKGTGNR.T
P15639	PurH	Bifunctional purine biosynthesis protein	806.3792 (2+)	2.80	72.7	K.NNMTIGIGAGQMSR.V
P0A9B2	GAPDH	Glyceraldehyde-3-phosphate dehydrogenase	1085.1689 (3+)	1.30	47	K.GANFDKYAGQDIVSNASCTTNCLAPLAK.V
Q9JMS5	YuaO	Uncharacterized protein	809.8575 (2+)	0.12	79.7	K.GNNASATLDYTDSK.I
			834.4002 (2+)	1.60	79.5	K.VSGNMSIAQGNAASAK.V
			802.8511 (2+)	1.60	74	R.GNNASATLDYSDSK.I
			854.9266 (2+)	−5.30	73.5	K.TQLELNNSALTASGK.V
			639.8131 (2+)	1.30	50.9	K.GNASIGVGDQAK.A
P39180	Ag43	Antigen 43	825.4345 (2+)	1.30	96.6	R.LQVDAGGTATNVTLK.Q
			654.8363 (3+)	0.82	33	K.TVNNDTLTIR.E
			878.9494 (2+)	5.80	57.4	K.SGSGTLTVSNTTLTQK.A
P22259	PCKA	Phosphoenolpyruvate carboxykinase	646.3407 (2+)	0.09	42	R.DALLENVTVR.E
P0A6E4	ArgG	Argininosuccinate synthase	665.3247 (2+)	0.04	33	K.DLEYLNSSVK.I
P37636	MdtE	Multidrug resistance protein	825.4197 (2+)	5.7	71.4	R.TQLNEAEANVTVAK.A