TABLE 1.
Differential proteins selected under TEA inducement identified by using MALDI-TOF-MS/MS
Mass spectrometric identification of differentially regulated proteins of HeLa cell under TEA inducement is shown. The table shows the gel-based IDs, protein names, Uniprot accession numbers, predicted mass and pI, protein score, and TEA/control change -fold. Only significant hits are determined by Mascot are considered as positive identifications.
Spot no. | Protein name | Accession no. | Mr (Da)/pI | Protein score | Relative intensity ratio | Change |
---|---|---|---|---|---|---|
1 | POTE ankyrin domain family member E | Q6S8J3/POTEE | 122882.3/5.83 | 271 | 1.43 | ↑ |
2 | Seryl-tRNA synthetase, cytoplasmic | P49591/SYSC | dinner 59253.2/6.05 | 376 | 0.56 | ↓ |
3 | T-complex protein 1 subunit α | P17987/TCPA | 60818.8/5.8 | 644 | 0.11 | ↓ |
4 | Protein disulfide isomerase A3 | P30101/PDIA3 | 57145.9/5.98 | 831 | 0.76 | ↓ |
6 | 60 S acidic ribosomal protein P0 | P05388/RLA0 | 34422.9/5.71 | 120 | 0.56 | ↓ |
7 | Dienoyl-CoA isomerase, mitochondrial | Q13011/ECH1 | 36135/8.16 | 699 | 2.31 | ↑ |
8 | Glutathione S-transferase ω1 | P78417/GSTO1 | 27833.2/6.23 | 393 | 0.82 | ↓ |
9 | Enoyl-CoA hydratase, mitochondrial | P30084/ECHM | 31823.3/8.34 | 285 | 1.49 | ↑ |
10 | Platelet-activating factor acetylhydrolase IB subunit γ | Q15102/PA1B3 | 25832.2/6.33 | 160 | 0.58 | ↓ |
11 | Proteasome subunit α type-6 | P60900/PSA6 | 27838/6.34 | 380 | 1.38 | ↑ |
12 | Triosephosphate isomerase | P60174/TPIS | 26937.8/6.45 | 79 | 0.35 | ↓ |
13 | BAG family molecular chaperone regulator 2 | O95816/BAG2 | 23928.3/6.25 | 81 | 1.23 | ↑ |
14 | Acyl-protein thioesterase | O75608/LYPA | 24995.6/6.29 | 100 | 1.26 | ↑ |
15 | Protein DJ-1 | Q99497/PARK7 | 20049.6/6.33 | 250 | 0.62 | ↓ |
16 | Thioredoxin-dependent peroxide reductase, mitochondrial | P30048/PRDX3 | 28017.3/7.67 | 247 | 0.058 | ↓ |
17 | Proteasome subunit β type 4 | P28070/PSB4 | 29242.5/5.72 | 340 | 1.45 | ↑ |
18 | Heat shock protein β1 | P04792/HSPB1 | 22825.2/5.98 | 534 | 0.063 | ↓ |
19 | Translocon-associated protein subunit δ | P51571/SSRD | 19157.7/5.76 | 347 | 0.75 | ↓ |
20 | Prefoldin subunit 6 | I15212/PFD6 | 14573.8/8.83 | 59 | 0.34 | ↓ |
21 | ATP synthase subunit d, mitochondrial | O75947/ATP5H | 18536.5/5.21 | 194 | 0.37 | ↓ |
22 | Chromobox protein homolog 3 | Q13185/CBX3 | 20969.4/5.23 | 197 | 1.21 | ↑ |
23 | Ferritin heavy chain | P02794/ FRIH | 21383.4/5.3 | 137 | 0.074 | ↓ |
25 | Sorcin | P30626/SORCN | 21947.5/5.32 | 121 | 0.12 | ↓ |
26 | NADH dehydrogenase iron-sulfur protein 8, mitochondrial | O00217/NDUS8 | 24202.8/6 | 147 | 0.24 | ↓ |
27 | Ran-specific GTPase-activating protein | P43487/RANG | 23466.6/5.19 | 241 | 0.13 | ↓ |
28 | Rho GDP-dissociation inhibitor 1 | P52565/GDIR1 | 23249.7/5.02 | 367 | 0.52 | ↓ |
29 | EF-hand domain-containing protein D2 | Q96C19/EFHD2 | 26794.5/5.15 | 77 | 0.36 | ↓ |
30 | Tubulin-folding cofactor B | Q99426/TBCB | 27593.6/5.06 | 82 | 0.35 | ↓ |
31 | U3 small nucleolar RNA-associated protein 15 homolog | Q8TED0/UTP15 | 58660.6/9.18 | 44 | 0.33 | ↓ |
32 | Diphthine synthase | Q9H2P9/DPH5 | 31973.4/5.19 | 73 | 1.73 | ↑ |
33 | Heterogeneous nuclear ribonucleproteins C1/C2 | P07910/HNRPC | 33706.6/4.95 | 147 | 1.83 | ↑ |
34 | Stress-70 protein, mitochondrial | P38646/GRP75 | 73919.9/5.87 | 650 | 1.15 | ↑ |
35 | Heat shock 70-kDa protein 1A/1B | P08107/HSP71 | 70294.1/5.48 | 975 | 1.82 | ↑ |