TABLE 1.

Recycling kinetics of ß₁-AR and ß₂-AR chimera in HEK-293 cells

Recycling β-AR construct	Additional conditions	t0.5 for recycling	Statistical significance
		min
WT β1-AR		21 ± 6
WT β1-AR	+3 μm H-89/mPKI	No recycling	NDa
WT β1-AR	+Scrambled shRNA	22 ± 8	p > 0.05b
WT β1-AR	+SAP97 shRNA	No recycling
(K476L) β1-AR		24 ± 7	p > 0.05b
(E474D) β1-AR		38 ± 9	p < 0.01b
(V477I) β1-AR		19 ± 7	p > 0.05b
(V477L) β1-AR		41 ± 10	p < 0.01b
WT ß2-AR		25 ± 6
WT β2-AR	+Scrambled shRNA	27 ± 7	p > 0.05c
WT β2-AR	+SAP97 shRNA	28 ± 8	p > 0.05c
WT β2-AR	+3 μm H-89	23 ± 7	p > 0.05c
WT β2-AR	+3 μm mPKI	25 ± 8	p > 0.005
(S312D) β1/β2-AR(10)		20 ± 7	p > 0.05b
(S312D) β1/β2-AR(10)	+Scrambled shRNA	21 ± 7	p > 0.05b
(S312D) β1/β2-AR(10)	+SAP97 shRNA	23 ± 8	p > 0.05b
(S312D) β1/β2-AR(10)	+3 μm H-89	25 ± 8	p > 0.05b
(S312D) β1/β2-AR(10)	+3 μm mPKI	24 ± 7	p > 0.05b
β1-AR aquaporin(10)		25 ± 8	p > 0.05b
β1-AR aquaporin(10)	+Scrambled shRNA	29 ± 9	p > 0.05b
β1-AR aquaporin(10)	+SAP97 shRNA	No recycling
β1-AR GluR1(10)		22 ± 7	p > 0.05b
β1-AR GluR1(10)	+Scrambled shRNA	27 ± 9	p > 0.05b
β1-AR GluR1(10)	+SAP97 shRNA	No recycling
β1-AR μ-opioid(12)		21 ± 6	p > 0.05b
β1-AR μ-opioid(12)	+3 μm H-89	20 ± 6	p > 0.05b
β1-AR GluR2(10)		20 ± 6	p > 0.05b
β1-AR GluR2(10)	+3 μm H-89	22 ± 7	p > 0.05b
β1-AR dopamine R1		27 ± 8	p > 0.05b
β1-AR dopamine R1	+3 μm H-89	30 ± 11	p > 0.05b

^a Not determined.

^b Relative to t_0.5 of WT β₁-AR recycling.

^c Relative to t_0.5 of WT β₂-AR recycling.