FIGURE 6.

Calculated [NAD⁺]/[NADH] from fitted [1-¹³C]lactate/[1-¹³C]pyruvate ratios. A, [[1-¹³C]lactate/[1-¹³C]pyruvate ratios obtained from fitted [1-¹³C]lactate and [1-¹³C]pyruvate signals in MCF7 and PC3 cells. Significant ratio values between the two cell types were evaluated with Student's t test, indicated by *, p < 0.0001. B, calculated [NAD⁺]/[NADH] ratios using the lactate dehydrogenase (LDH) equilibrium equation, at pH 7.0 for MCF7 cells and pH 7.1 for PC3 cells and K_eq = 1.11 × 10⁻¹¹ m¹⁰.