FIGURE 5.
Effect of the αIIb β-propeller-derived peptides on platelet adhesion. Microtiter wells were coated with 10 μg/ml D98 fragment and postcoated with 1% BSA. 50-μl aliquots containing different concentrations of the αIIb-derived peptides in isotonic HEPES buffer were added to the wells for 15 min at 37 °C followed by suspensions of calcein-labeled platelets (1 × 107/50 μl). After 30 min at 37 °C, nonadherent cells were removed, and adhesion was determined. A, a dose-dependent inhibition of adhesion by αIIb 94–108 is shown. B, inhibition of platelet adhesion by each peptide used at 250 μm is shown. The data are expressed as the percentage of adhesion in the absence of peptides. Error bars represent S.D. The results shown are the average of triplicate measurements at each experimental point and are representative of three to five experiments.