FIGURE 3.

hESCs with JMJD1C knockdown remain pluripotent. A, immunostaining for OCT4 or NANOG on JMJD1C KD or MM H9 cells cultured in either CM or T1 medium. Cell nuclei were counterstained with DAPI. B, JMJD1C KD and MM H9 cells were injected into SCID-beige mice. Teratomas exhibiting complex differentiation formed in the mice 5–6 weeks postinoculation and were processed for histological analysis with hematoxylin-eosin staining. Representative images for the three germ layers are shown. C, PluriTest. Two JMJD1C KD, two MM, and one wild-type hESC sample (for both H9 and CT2) were subjected to PluriTest via analysis of their gene expression microarray data, and all of the samples (shown as small circles in the graphs) passed the test.