FIGURE 6.

miR-302 reduction contributes to neural differentiation in hESCs with JMJD1C knockdown. A, phase-contrast photographs of the JMJD1C KD H9 cells transfected with a miR-302c mimic or a negative control (Neg. Ctrl.) and cultured in T1 or T1/F0 for 5 days. Note that neural rosettes were only present in the KD cells transfected with the negative control mimic but not the miR-302c mimic. B–D, flow cytometry analysis for the ratio of PAX6⁺ cells among H9-KD cells transfected with the miR-302c mimic (B) or a miR-302b mimic (C), wild-type H9 cells transfected with a miR-302c inhibitor (Inh.), or a miR-302b inhibitor (D). E, a model for JMJD1C-miR-302-mediated inhibition on neural differentiation of hESCs. Arrows, induction; hammer-ended line, inhibition.