TABLE 2.
Primers |
||
---|---|---|
Forward | Reverse | |
RT-PCR and -qPCR | ||
JMJD1C | CCAAAATTGCCTGGAAAAGA | ACATCACTCTCTGGGCTGCT |
OCT4 | GACAACAATGAGAATCTTCAGGAGA | CTGGCGCCGGTTACAGAACCA |
NODAL | GGGCAAGAGGCACCGTCGACATCA | GGGACTCGGTGGGGCTGGTAACGTT |
PAX6 | TGTCCAACGGATGTGTGAGT | TTTCCCAAGCAAAGATGGAC |
NR2F2 | GCCATAGTCCTGTTCACCTCA | AATCTCGTCGGCTGGTTG |
TGFBR2 | GCTACTCCATGGCTCTGGT | ATCTGGATGCCCTGGTGGTT |
Pri-miR-302 | GGAGAACACGAATCTTTGGG | GAAAGAGTATTTGGGAAATGT |
GAPDH | TCTTTTGCGTCGCCAGCCGAG | TCCCGTTCTCAGCCTTGACGGT |
ChIP-PCR and -qPCR | ||
Region 1 | GAAACACAATGCCTTTCTCG | TCTGCTTGTTTGATGGCATAG |
Region 2 | TCTTGTGGTAATGGTTTTAGCTG | GGTGGGTCTGGATCCTTTTT |
Region 3 | CAAAAGGCCCTTGTAATGATTC | GGGTAAAAGGCAGGGACTTC |
Region 4 | GAACACGCTAACCTCATTTG | GACACCTCCACTGAAACATG |
Region 5 | CTTGGAAGCTTCTGATGC | CAGCTCTGAAGATGCAGAATC |