FIGURE 6.

ApoA-IV represses Glc-6-Pase and glucose production through NR1D1. Primary mouse hepatocytes were transfected with siRNA against mouse NR1D1 (siNr1d1) or control siRNA (siCon) for 48 h and then treated with or without 20 μg/ml r-m-apoA-IV for 6 h or overnight as described in Fig. 3. A, NR1D1 protein detected by Western immunoblotting to determine the magnitude of inhibition of protein expression. B, glucose in the medium after NR1D1 inhibition. ***, p < 0.001 versus control siRNA. C and D, mRNA levels of Glc-6-Pase (C) and PEPCK (D) were quantitated by real-time RT-PCR and normalized to cyclophilin. ns, not significant. E, glucose in the medium. Data are presented as mean ± S.E. from three independent experiments with at least three samples each time. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus vehicle of control siRNA.