FIGURE 7.

ApoA-IV induces NR1D1 expression in primary mouse hepatocytes and HepG2 and HEK-293 cells. The cells were starved in DMEM with 1% BSA overnight and then treated with or without 20 μg/ml r-m-apoA-IV in mouse hepatocytes for the indicated times or 50 μg/ml r-h-apoA-IV in HepG2 and HEK-293 for 2 h. mRNA levels in primary mouse hepatocytes were quantitated by real-time RT-PCR and normalized to cyclophilin. NR1D1 protein levels were detected by Western immunoblotting with anti-NR1D1 antibody and then standardized against actin. The results are from three independent experiments with at least three samples each time. Data are presented as mean ± S.E. *, p < 0.05; **, p < 0.01 versus vehicle. A and B, NR1D1 mRNA level (A) and NR1D1 protein level (B) in primary mouse hepatocytes. C, NR1D1 protein level in HepG2 cells. D, NR1D1 protein level in HEK-293 cells.