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Effect of ES with liposomes on intercellular junctions of cultured cells in vitro. Immunohistochemical analysis of tight junction proteins ZO-1 (green) and CLD-4 (red) phosphorylated Cx43 (green) and F-actin (red) in (A) NHEK cells or (B) Caco-2 cells after ES treatment with/without liposomes. Bar indicates 20 μm. Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, blue).
