Figure 5. Cell viability assay.
104 A431 or HeLa cells were transduced with 3 × 108 genomic viral particles. After 48 h fresh medium containing 5-FC was added to the cells. On day six an MTT assay was carried out and the amount of reduced formazan quantified. Values were calulated relative to the cells-only signal and then the latter was substracted to reflect the decrease in viability.