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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: Nature. 2013 Aug 11;501(7467):435–438. doi: 10.1038/nature12467

Figure 1. Tissue distribution of VLRA+, VLRB+ and VLRC+ lymphocytes.

Figure 1

a, Flow cytometric analysis of lymphocyte-gated cells stained with monoclonal antibodies specific for VLRA (R110), VLRB (4C4) and VLRC (3A5) (left). Lymphocyte population in lamprey larvae (right); n = 11.

b–g, Immunofluorescence staining of VLRC+ (green) and VLRA+ (red) lymphocytes in larval tissue sections, and DAPI counterstaining of nuclei (blue); scale bars, 50 μm (b–e, g). Shown are typhlosole surrounded by intestinal epithelium (intraepithelial lymphocytes) (b, arrows), kidneys (c), gill filaments (d; inset is a magnification of the area indicated by the dashed box), hypopharyngeal fold (e), skin (g). Lymphocyte distribution in the intestinal epithelium and skin epidermis is shown in f; n = 5 larvae. ***P < 0.0001; error bars, s.e.m. h, Frequency of replicate VLRC sequences in indicated tissues from two larvae; replicate VLRC sequences were not shared by different tissues. i, Frequency of VLRA replicates. The numbers of clonal replicates are colour-coded: red, 22; blue, 7; green, 5; pale orange, 4; yellow, 3; orange, 2.