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. 2012 May 10;1(2):127–140. doi: 10.3390/cells1020127

Figure 3.

Figure 3

Allo-antigen-specific T cells show dissociated IL-2-IFN-γ production. Allogeneic 20 h cultures were performed as 2-way MLR with 3 × 105 PBMC (panels A–C), or as a 1-way allogeneic response using CD3/CD56 depleted PBMC stimulators (300,000 cells/well) and CD4 (panels D–F), or CD8 (panels G–I) cell responders (300,000 cells/well). IL-2 sfu (panels A, D, G), IFN-γ sfu (panels B, E, H) and proliferation cpm (panels C, F, I) are shown. Stimulator identification is shown on the x-axis, while responder identification is shown in the legend. Shaded regions highlight discordance between IL-2 and IFN-γ producing effector function. Stimulator or responder cell alone control cultures resulted in <5 sfu and <1,000 cpm (not shown).