Figure 1.

Viability of cryopreserved peripheral blood mononuclear cells (PBMC) from seven subjects following thawing under warm or cold processing conditions. Warm wash medium was added to warm cells, and cold wash medium to cold cells, at a rate of 1 mL/5 seconds (slow) or in a single stream <5 seconds (fast), as specified in the Experimental Section. The viability of the cells was assessed by acrydin orange / ethidium bromide staining. Comparison of viability at various thawing conditions were performed by nonparametric Wilcoxon signed rank test with two-tailed p values ≤ 0.05 being considered significant; (ns) not significant.