Figure 2.

Viability and functionality of cryopreserved PBMC from 10 subjects following thawing under warm or cold processing conditions. (A) Post-thaw viability of PBMC under both conditions was assessed as described in Figure 1. (B) CD8 ELISPOT responses were measured using the CEF peptide pool that comprised of defined Class I peptides from CMV, EBV, and influenza viruses [18,19] as the antigen to stimulate the PBMC that were thawed according to the “warm-” or “cold processing protocol. (C). CD4 ELISPOT responses: mumps and mosquito antigen were used to stimulate CD4 cells. Statistical comparisons were performedwith Wilcoxon signed rank test with two-tailed p values ≤0.05 being considered significant.