Figure 3.

Endogenously activated autoreactive T cells produce either IFN-γ or IL-2, but not both simultaneously (double color, DC). Twelve days after the immunization with the PLP peptide, 1 × 10⁶ unseparated dLN cells, spleen cells, and spinal cord mononuclear cells were plated per well and tested in an IFN-γ /IL-2 double color ELISPOT assay of 48 h duration. Single and double color spots were counted by image analysis. Means and SD of spot forming cells for four independent experiments are shown, with triplicate wells in each experiment.