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. 2012 Oct 19;1(4):886–904. doi: 10.3390/cells1040886

Figure 2.

Figure 2

Rapamycin treatment does not affect TEAD1 protein expression levels but affects its nuclear localization in HepG2 cells. TEAD1 (green) expression does not change with and without rapamycin treatment at low and high cell density conditions. Bottom panels represent the boxes in top images: localization of TEAD1 at low and high cell density conditions demonstrates that, in contrast to MCF7 cells, it is mainly nuclear in low density conditions, while it is less nuclear at high density conditions. Rapamycin treatment increases nuclear localization at high density conditions. TOPRO3 (blue) labels nuclei. Scale bars, 20 µm.