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. 2012 Oct 19;1(4):886–904. doi: 10.3390/cells1040886

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure S2 — Rapamycin treatment does not affect YAP/P-YAP expression or localization in HepG2 cells. (A-B) Immunofluorescent staining of YAP (A, green) and P-YAP (B, green) in MCF7 cells with and without rapamycin treatment at low and high cell density conditions demonstrates no changes. Bottom panels represent box in top images: localization of YAP or P-YAP at low and high cell density conditions demonstrates cytoplasmic localization and does not change with rapamycin treatment. TOPRO3 (blue) labels nuclei. Scale bars, 50 µm.