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. 2012 Jun 1;2(2):366–381. doi: 10.3390/metabo2020366

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Analysis of the lipidome by GC-MS and LC-MS. A) A typical GC-MS chromatogram of fatty acid methyl esters in the organic fraction. Typical LC-MS data with identification. B) Total ion chromatogram of the LC-MS analysis of intact lipids. Phospholipids elute between 3.5 and 5.5 minutes and triacylglycerols between 6.5 and 8.5 minutes. C) Single scan mass spectrum from the LC-MS at 4.41 minutes D) extracted ion chromatogram for 748.5 E) MS-MS of 748.5 at 4.4 minutes. Characteristic fragments for a phosphoethanolamine with two fatty acids 22:0 and 22:6.