Table 4.
Changes in metabolite levels as detected by LC-MS in the combined gender model. Assignments were confirmed by MS/MS. Where individual fatty acids are not specified it was not possible to definitively identify the lipid beyond head group, total chain length and degree of unsaturation. Unknowns 1 and 2 are hydrophobic molecules with a retention time of 7.41 and 6.75 minutes and mass (M+NH4+) of 880.65 and 812.6; both of these unknowns have a fragment ion of 197 Da. Similar changes were detected in the individual gender models. Note it cannot be determined with certainty which fatty acids are in a given position from the fragmentation patterns.
| Increased in Knock out | Decreased in Knock Out | |
|---|---|---|
| LC-MS | PC(16:0/22:6) | PC(18:2/22:6) |
| PC(18:0/22:6) | PC(40:7) 2 isomers | |
| PE(22:6/20:4) | PC(36:4) | |
| PE(40:6) | PC(38:5) | |
| PE(22:0/22:6) | PC(36:3) | |
| PE(22:6/17:1) or PE(38:0) | PC(34:1) | |
| TAG(18:2/18:2/22:6) | PC(40:5) | |
| TAG(55:2) | PC(38:4) | |
| TAG(18:1/18:2/22:6) | PC(18:0/22:4) | |
| TAG(20:3/18:2/18:2) | PE(40:7) | |
| TAG(20:5/18:2/18:1) | PE(36:2) | |
| TAG(18:2/20:3/16:0) | PE(20:4/18:0) | |
| TAG(58:4) | PE(40:5) | |
| TAG(18:1/18:1/18:2) | PE(16:0/22:6) | |
| TAG(56:7) | Unknown 1 | |
| TAG(58:3) | Unknown 2 | |
| TAG(18:1/18:2/17:1) | ||
| TAG(54:3) mixture containing (16:0/18:1/18:2/18:0/20:1/20:2/20:3) | ||
| TAG(18:1/18:2/18:0) | ||
| TAG(18:1/18:1/20:1) |