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. 2012 Apr 20;2(2):337–365. doi: 10.3390/metabo2020337

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Targeted chiral lipidomics analysis of COX-2-derived eicosanoids from LoVo cells. LoVo cells were lysed; eicosanoids were extracted, derivatized with PFB bromide, and analyzed by LC-ECAPCI/SRM/MS. LoVo cell lysates were pretreated with 50 μM CAY10397 to inhibit 15-PGDH to be able to detect the 11-, 15-HETEs and PGE₂. Representative chromatograms are shown for (top to bottom) (a) 11(R)-HETE-PFB (m/z 319 → 167), (b) [²H₈]-15(S)-HETE-PFB internal standard (m/z 327 → 226), (c) 11-oxo-ETE-PFB (m/z 317 → 165) and 15-oxo-ETE-PFB (m/z 317 → 165), (d) [¹³C₂₀]-15-oxo-ETE-PFB internal standard (m/z 337 → 120), (e) PGE₂-PFB (m/z 351 → 271), (f) [²H₄]-PGE₂-PFB (m/z 355 → 275), (g) 13,14-dihydro-15-oxo-PGE₂-PFB (m/z 351 → 235), (h) [²H₄]-13,14-dihydro-15-oxo-PGE₂-PFB (m/z 355 → 239). Reprinted with permission from Ref. [110].