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. 2012 Apr 20;2(2):337–365. doi: 10.3390/metabo2020337

Figure 7.

Figure 7

LC-SRM/MS analysis and quantitation of 15-LOX-derived eicosanoids from R15L cells and RMock cells treated with arachidonic acid. A, representative chromatograms of 15-LOX-derived lipid metabolites released by R15L cells after 5-min treatment with 10 μM arachidonic acid. SRM chromatograms are shown for 15-oxo-ETE-PFB (m/z 317 → 273) (a), [2H6]5-oxo-ETE-PFB internal standard (m/z 323 → 279) (b), 15-(R,S)-HETE-PFB (m/z 319 → 219) (c), and [2H8]15-(S)-HETE-PFB internal standard (m/z 327 → 226) (d). B, concentration-time graph of 15-HETE (R- and S-form) released by R15L or RMock cells treated with 10 μM arachidonic acid for 24 h. C, concentration-time graph of 15-oxo-ETE released by R15L or RMock cells treated with 10 μM arachidonic acid for 24 h. Cell supernatants were collected at each time point. Lipid metabolites in the cell supernatants were extracted and derivatized with PFB. Determinations were conducted in triplicate (means ± S.E.M.) by stable isotope dilution chiral LC-ECAPCI/SRM/MS analyses of PFB derivatives. Reprinted with permission from Ref [108].