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Diagram representing different domains present in HS chains. Sulphated domains (S-domains) contain N-sulphated disaccharides with IdoA-2-O- sulphate as a major uronate component. N-acetylated (NA) domains are non-sulphated and have acetylated regions. In contrast, N-acetylated and N-sulphated (NA/NS) domains contain alternating N-acetylated and N-sulphated units. The cleavage sites for commonly used heparitinase digestion enzymes are shown [11].
