Evaluation of S. mutans adherence to SAG and detection of anti-S. mutans IgG. (A) BIAcore SPR analysis was used to evaluate bacterial adherence following incubation of S. mutans with sera collected from mice immunized with full-length P1 (CG14) and truncated derivatives of P1 (NA1, P3C). Values were normalized to the change in resonance units (ΔRU) for S. mutans NG8, in the absence of added serum, detected following the 60-second injection cycle. Immunogens are indicated on the X-axis. Tukey’s Multiple Comparison Test determined differences among the groups; *p≤0.05, **p≤0.01, ***p≤0.001, ****p≤0.0001 (B) The presence of anti-S. mutans IgG in the sera of mice immunized with P1 versus truncated P1 polypeptides was evaluated by serial dilution and standard ELISA. Data are representative of at least three independent experiments. Statistical significance between groups was evaluated by two-way ANOVA and no significant differences were observed.