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Kinetics of GPx7 oxidation by H2O2. (A) Stopped-flow trace of time-dependent changes in the intrinsic fluorescence of 10 μM GPx7 on addition of 1 mM H2O2. The initial reaction fits well to a pseudo-first-order reaction (inset). (B) Linear dependence of the pseudo-first-order rate constant for GPx7 oxidation on the concentration of H2O2 (n≥3).
