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. Author manuscript; available in PMC: 2014 Jan 24.
Published in final edited form as: J Immunol. 2010 Dec 3;186(1):312–322. doi: 10.4049/jimmunol.1001989

FIGURE 2.

FIGURE 2

CD4+ T cell responses to immunodominant LAGE-1–de-rived peptides. CD4+ T cells isolated from PBLs of melanoma patients (n = 7) were stimulated weekly with irradiated autologous mature DCs pulsed with pooled LAGE-1 peptides prior to testing reactivity against each of the six LAGE-1 peptides and the irrelevant peptide LAGE-1 ORF285–102 in IFN-γ ELISPOT assays. A positive response is defined as mean of spot numbers (triplicate) obtained for a given LAGE-1 peptide greater than the mean of spot numbers obtained for the irrelevant peptide LAGE-1 ORF285–102 + 3 SD and greater than 20 spots per 104 CD4+ T cells. The means of spot numbers obtained for the irrelevant peptide LAGE-1 ORF285–102 (i.e., background) were always <50/104 CD4+ T cells. Each depicted symbol represents the mean of spot numbers (triplicates) observed in the presence of a given LAGE-1 peptide minus the mean of spot numbers in the presence of the irrelevant peptide LAGE-1 ORF285–102 (i.e., background). Spots were developed and counted by computer-assisted video image analysis. One representative experiment of three performed is depicted.