A–F. Immunostaining for BrdU (Green), PECAM (Red) and DAPI (Blue) in the adult prostate of saline-instilled control (A), 3 days post-bacterial inoculation (B), 5 days post-bacterial inoculation (C), 7 days post-bacterial inoculation (D), postnatal day 15 (E) and adult (F) prostate. Scale bar 100μm. G. Comparisons of the endothelial proliferation determined by double positive PECAM/BrdU (+/+) cells per 20X field in the prostate after saline instillation (n=5) and day 3, 5, 7 post-bacterial inoculation (n=4 each time point). The data are presented as the mean ± SEM. * indicates p-value < 0.05 compared to saline by two-sample t-test. H. Comparison of endothelial proliferation determined by double positive PECAM/BrdU cells per 20X field in the prostate of postnatal day 15 (n=6) and adult (n=6) male mice. The data are presented as the mean ± SEM. * indicates a p-value < 0.05 compared to adult by two-sample t-test. PID Post instillation day.