FIG. 2.
BBR inhibited NO release and ROS production in LPS-stimulated macrophages. (A) RAW264.7 cells were treated with LPS (10 ng/ml), BBR (5 μM, 1 h earlier when used in combination) or both for 24 h. NO concentration in culture supernatant was measured and expressed as percentage values, taking the LPS treatment group as 100%. (B) After treatment with LPS or/and BBR, cells were washed and labeled with H2-DCFH-DA probe. A set of representative images showing fluorescent ROS was shown. (C) Quantitative measurement of cellular ROS was performed by flow cytometry after H2-DCFH-DA staining. Similar results were obtained from three independent experiments. *p<0.05 and **p<0.01. DCFH-DA, 2′, 7′-dichlorodihydrofluorescein diacetate; ROS, reactive oxygen species. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars