FIG. 6.

Blockage of AMPK signaling abolished suppressive effects of BBR on inflammation and oxidative stress in RAW 264.7 cells. (A) Cells were preincubated with or without compound C (CC; 2 μM) and BBR (5 μM), then treated with LPS (10 ng/ml). Whole cell lysates were subjected to Western-blot analysis with antibodies against phosphoylate-AMPKα1 at Thr172 (p-AMPKα1) and total AMPKα1. (B) After treatments as in A, cellular concentrations of AMP and ATP were determined through high performance liquid chromatography. (C) After treatments as in A, mRNA levels of indicated genes were monitored by qRT-PCR. Results are means±SEM from three independent experiments. **p<0.01.