Figure 6. MGL knockdown enhances phosphorylation of Akt.
HT29 (A) and MDA-MB-231 (B) colon and breast cancer cells, respectively, were infected with lentivirus carrying MGL shRNA or scramble shRNA. Seven to ten days after infection, cells were treated with 100 μg/ml insulin 30 min prior to being lysed. Western blot analyses were performed sequentially on the same membrane with indicated antibodies.