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. 2013 Aug 20;2:147. Originally published 2013 Jul 4. [Version 2] doi: 10.12688/f1000research.2-147.v2

Figure 2. Three-dimensional confocal reconstructions of z stacks through cartilage explants (15μm sections) after five days in culture.

Figure 2.

Control ( A) consists of culture media with 2% penicillin/streptomycin. Sodium nitroprusside (SNP) (50mM) ( B) is a positive control for cell death. Treatments consist of curcumin in culture media at 25μM ( C) and 100μM ( D). Images are representative of explants in the treatments wells for each animal. Green staining indicates live metabolizing cells and red staining highlights the nuclei of dead cells. Data are from 3 different animals, with one technical replicate per animal.