Table 1. List of primers used for Thermal Asymmetric InterLaced (TAIL) PCR, verification of TAIL-PCR product and DNA sequencing.
Primer name | Sequence of primer | Location |
---|---|---|
AD | 5´-NTC GWG WTS CNA GC-3´ | Random degenerate primer |
AD2 | 5´-ATCGTGTTCCCAGC-3´ | Non-degenerate primer derived from the primer AD |
1F | 5´-AAA GAC TGA TCA GCA CGA AAC GGG-3´ | APHVIII 3´UTR |
2F | 5´-TAA GCT ACC GCT TCA GCA CTT GAG-3´ | APHVIII 3´UTR |
2R | 5´-CTC AAG TGC TGA AGC GGT AGC TTA-3´ | APHVIII 3´UTR |
3R | 5´-TCT TCT GAG GGA CCT GAT GGT GTT-3´ | APHVIII 3´UTR |
4R | 5´-GGG CGG TAT CGG AGG AAA AGC TG-3´ | APHVIII 3´UTR |