Skip to main content
. 2013 Jul 29;2:138. Originally published 2013 Jun 10. [Version 2] doi: 10.12688/f1000research.2-138.v2

Figure 10. Molecular analysis of chli1-1 rescued transformants.

Figure 10.

( A) A schematic of the native CHLI1 gene. The tan bars denote UnTranslated Regions (UTRs), the white arrows represent exons and the black lines denote introns. ( B) A schematic of the CHLI1-pDBle complementation vector containing the CHLI1 cDNA. PsaD promoter, 5´UTR, 3´UTR and CHLI1 specific primers are labeled. ( C) Genomic DNA PCR using a PsaD 5´UTR specific primer and a CHLI1 specific primer. Product size: 1272 bp. Lane 1: chli1-1; Lane 2: 4A+; Lane 3: chli1-7; Lane 4: chli1-8. ST represents 1 kb plus DNA ladder. ( D) Genomic DNA PCR using CHLI1 specific primers. Genomic DNA product size: 459 bp; cDNA product size: 249 bp. Lanes 3 and 4 show smaller PCR products compared to that in lane 2 as cDNA was used for complementation. Lane 1: chli1-1; Lane 2: 4A+; Lane 3: chli1-7; Lane 4: chli1-8. ST represents 1 kb plus DNA ladder. All primer sequences are shown in Table 4.